Abstract:
The green synthesis of metal oxide nanostructure is an interesting subject of nanoparticles. Also, of latest concern is the biosynthesis of metal oxide nanostructure using plants for the large-scale biosynthesis. And doping lead to versatile changed properties as result of slightly changed structure. Therefore, the main aim of the present study was green synthesize of Ni 2+ doped ZnO nanostructure to evaluate their antimicrobial activity against some selected microbes. The sample was prepared from nickel nitrate hexahydrate and Zinc nitrate hexahydrate with extracted Euphorbia abyssinica bark plant at roomtemperature. This method was interested to synthesize Ni doped ZnO nanostructureusing Euphorbia abyssinica bark plant extract; since the process of synthesis was eco-friendly, low cost, quite suitable for biological and medical application.Finally the synthesized samples had been characterized and examined by UV-Vis spectrum, (FT-IR) Fourier Transforms Infrared and x-ray diffraction (XRD).The UV-Vis maximum absorption peak shows that as doping increase the lambda max increases as result of new atom insertion, from FTIR the stretching vibration of the Ni doped ZnO ranges between 524 to 439 nm and XRD patters showed the crystallite of Ni doped and un doped ZnO Nps and using Debye-scherrer formula, the size of the sample was determined. As doping increase the size was increased from 8.11 nm to 14.90 nm and the band gap was decreased. in this work using green methods nano sized with simple procedure well crystal sample was synthesized and Lastly it was investigated the antimicrobial activity of Ni 2+ doped ZnO nanostructure with gram positive and gram negative bacterial strains and antifungal activity by agar well diffusion method and their inhibition zone showed good anti-bacterial and anti-fungal activity.