reference interval establishment for selected clinical chemistry parameters for apparently health individuals with age greater than or equal to five years in south west Ethiopia

Abstract

Background:Reference Intervals are the result of quantitative analysis of a clinical chemistry parameters, obtained from a group of apparently health individual or group of individuals who are selected according to the clearly defined criteria of International Federation Of Clinical Chemistry/Clinical Laboratory Science Institute(IFCC/CLSI )recommend. Selecting reference individuals are an essential but difficult step in the production of RIs throughout the world. As the diversity of the global population increases, a serious need exists to develop meaningful and reliable RI that can be used across regions and population groups. Objective: To determine Reference Interval of selected clinical chemistry parameters for apparently healthy individuals with age ≥5 years and to show sex and age difference in reference intervals in south west, Ethiopia. Methods: A community based cross sectional study was conducted by involving a total of 998 apparently healthy male and female subjects of age 5-71years from the three town with the ratio of their population and the study was conduct from march 2017-May 2017G.C.A serum sample was used for clinical chemistry parameters analysis by using HUMANSTAR-100 analyzer(HUMAN, Germany). Control samples were used for every test batch for all parameters. RIs were constructed using non-parametric methods to estimate 2.5 and 97.5 percentiles of distribution as lower and upper reference limits, respectively. Data were entered using Epi-data and analyzed using SPSS version 20 software. Mann Whitney U test and Kruskal Wallis test were used to show sex and age difference respectively. Result: There were significant differences in relation to gender in all age group RI for aspartate aminotransferase, Alanine aminotransferase, alkaline phosphatase, urea and creatinine. There were also significant age differences between all age group in the RI of both sex for aspartate aminotransferase and Alanine aminotransferase. Conclusion:. The findings of this study provide sex and age specific Reference Intervals for age >=5 years from south west Ethiopian region. Some parameters of this study show sex and age difference and also a difference between other countries and reagent providers RI. Adoption of this RI is recommended in this region and determination of similar RI for other regions in Ethiopia