Green synthesis, characterization and antimicrobial study of persicaria lapathifolia seed extract supported silver nanoparticles

Abstract

Multi-drug resistance is a growing problem in the treatment of infectious diseases. The widespread use of antibiotics has produced resistance for many human bacterial pathogens. The advancement of nanotechnology, indicated bio-synthesis of nanoparticles supported on medicinal plants have improved therapeutic capacity Silver nanoparticles. Medicinal plants are parts of plant that have medicinal purposes. Preliminary phytochemical investigations of Persicaria lapathiofolia crude extract has showed the presence of different secondary metabolites as of flavonoids, Alkaloids and phenolic compounds. The main objectives of this study is to synthesize, characterize and evaluate antimicrobial study of Persicaria lapathifolia seed extract supported silver nanoparticles. The synthesized Silver nanoparticles were characterized by UV-Vis, FT-IR spectroscopy, Powder XRD analysis, and TEM images. The green synthesized Silver nanoparticles were characterized by color change pattern, and the broad peak at 413 nm in the UV–Vis region. The observed surface plasmon resonance suggested formation of small sized Silver nanoparticles. The color change observed during preliminary phytochemical investigation and the FT-IR spectroscopic analysis suggested flavonoids, phenols and Alkaloids are highly responsible for reduction and stabilization of the synthesized silver nanoparticles. From the crystallographic studies of XRD and images of TEM microscopic studies, the synthesized Silver nanoparticles were polycrystalline in nature owing to 18.9 nm and spherical in shape. Finally, the bio-assay test showed the highest antimicrobial activity of Silver nanoparticles than plant extract against Escherichia coli from rest of bacterial strains as a result of synergistic effect.