Synthesis of ag2s-cuo–zno ternary Nano composite for determination Of ascorbic acid and antioxidant activity

Abstract

Ternary nanocomposite has received considerable attention owing to their novel characteristics and potential applications on ascorbic acid determination and antioxidant activity. The aim of this study was to synthesize, characterize, conduct electrochemical determination of ascorbic acid and evaluate the antioxidant activity of Ag2S-CuO-ZnO ternary nanocomposite. Ag2SCuO−ZnO nanocomposite was synthesized using a facile and cost-effective homogenous precipitation method and it was done by mixing CuO−ZnO and AgNO3 precursors in 60 mL of ethanol and ultrasonicated for 40 min. The synthesized Ag2S-CuO-ZnO ternary nanocomposite was characterized by UV-VIS, FT-IR, XRD, SEM and CV. Ascorbic acid showed an irreversible oxidation peak. While the observed shift in peak potential with pH in the range of 3 to 7 indicated the participation of protons during ascorbic acid oxidation, the change in peak potential shift with scan rate in the range of 10 to 120 mV / s confirmed the irreversibility of the oxidation reaction. A better linear oxidative peak current on ascorbic acid was observed in the range of 10-150 μM with (LOD) and (LOQ) of 1.37 μM and 4.59μM, respectively. Recovery in the range (96 -105%) for spiked standard ascorbic acid in orange confirmed the potential applicability of the method for the determination of ascorbic acid in real samples. The Ag2SCuO-ZnO nanocomposite showed better antioxidant activity than CuO-ZnO confirmed with IC50 value of 560µg/ml and 881µg/ml, respectively while that of reference standard ascorbic acid is 98µg/ml. Due to this, Ag2S-CuO-ZnO nanocomposite revealed promising antioxidant activity compared to standard ascorbic acid. Therefore, the synthesized Ag2S-CuO-ZnO nanocomposite was important for determination of ascorbic acid and antioxidant activity.