Evaluation Of The Effect Of Methanol Crude Extract Of Juglans Regia Leaf On Liver Enzymes And Lipid Profiles Of Swiss Albino Mice Fed High Fat Diet

Abstract

Background: High fat diets feeding an important risk factor for obesity which accelerate over production of reactive oxygen species in hepatocytes cells and debilitating cellular antioxidant capacity that aggravate metabolic disturbances such as hyperlipidemia and liver diseases through increased of liver enzymes and lipid profiles. Juglans regia is a plant used by community to treat hyperlipidemia, high blood pressure and liver diseases, and its ingredients have antioxidant role through scavenge of accumulated fatty in hepatocytes. Thus, the current study intended to evaluate the effect of Juglans regia extract on liver enzymes and lipid profiles of mice fed high fat diet. Methods: Study was conducted at Jimma University Tropical and Infectious Disease Research Center (JUTIDRC) and thirty male Swiss albino mice were randomly divided into six groups. Group I (normal control ) were given distilled water, Group II were given HFD only, Group III were received atorvastatin, G- IV,V and VI of mice were received HFD and 100, 200 and 300mg/kg of Juglans regia crude leaf extract, respectively for thirty days. All mice were fasted overnight and sacrificed by cervical dislocation and 2-2.5ml of blood was drawn to evaluate lipid profiles and liver function tests, and liver histopathology was investigated. Data was entered into Epi-data 3.1 & exported to SPSS 25 software for analysis by using one way ANOVA. Results: Serum level of ALP, AST and TG of mice fed high fat diet (G-II) were significantly elevated (P<0.01) when compared to normal control group (G-I).Group of mice treated with atorvastatin (G-III) and Juglans regia crude leaf extract at high dose (300mg/kg) significantly decreased (P<0.05) elevated AST, TG, TC and ALP when compared to group of mice fed high fat diet only (G-II). Furthermore, the liver section of a group of mice fed HFD (G-II) suffered moderate cell necrosis, vacuolar degeneration and mild-mixed inflammation when compared to normal control group, despite such abnormalities being absent in G-III and G-VI.