Abstract:
Municipal waste is a major environmental problem in developing countries.. However,
microbial enzymes can help reduce its negative impact. To this end the enzymatic activates
of solid waste microbes were evaluated, accordingly. A total of 12 sold waste samples were
collected from four area (three from each) and analysed for microbial and physiochemical
properties. Results of the study showed pH (7.6-–7.833 ±0.12 ), moisture content (1.09 ±
0.10) were significantly higher (P< 0.05) compared to control soil samples. Similarly,
dumpsite soil organic matter was significantly (P<0.05) increased with increased waste
dumping moisture content. the mean microbial counts (CFUg-1) were dominated by aerobic
mesospheric bacteria count (6.8 ) CFU g-1, S. aurous (4.4 ± 0.5) CFU g-1,
Enterobacteriaceae (2.96 ± 0.1 CFU g-1), fungi (2.3 ) CFU g-1,the count of Coli form
bacteria were 1.96 log10 CFU g-1 and 3.2 log10 CFU in area 2 and 3 samples, respectively
.Among ..the total 120 isolates 18 Fungi and 102 bacterial isolates are identified as
bacterial isolates. Bacillus spp. were the most prevalent (43.7%) among others followed by
Staphylococcus spp. (24%), Pseudomonas spp. (8%), Acinetobacter spp. (6%) and Coli
form (3%) The crude enzyme activity and stability study revealed that the amylase is stable
within the pH 6 – 8phvalus and temperature 30˚C - 40˚Cwith the maximum activity at
37˚Ctempereture and 8 pH value. In this study, temperature and moisture content had
major roles on the activity of amylase., the microbial quality of soil associated with
municipal solid waste of Bedele town was poor, particularly Among the the four isolates
BI16,BI5,FIA,FIH exhibited amylase production, FIH selected for amylase production with
high titer value. These results have increased the scope of finding industrially important
bacteria from municipal waste dump sites and FIH isolates could be vital source for the
discovery of industrially useful enzymes.
Keywords: waste dump sites, bacteria, , extracellular enzyme
