Evaluation of the enzymatic activities of microbial associated with solid waste dump site in Bedele town

Abstract

Municipal waste is a major environmental problem in developing countries.. However, microbial enzymes can help reduce its negative impact. To this end the enzymatic activates of solid waste microbes were evaluated, accordingly. A total of 12 sold waste samples were collected from four area (three from each) and analysed for microbial and physiochemical properties. Results of the study showed pH (7.6-–7.833 ±0.12 ), moisture content (1.09 ± 0.10) were significantly higher (P< 0.05) compared to control soil samples. Similarly, dumpsite soil organic matter was significantly (P<0.05) increased with increased waste dumping moisture content. the mean microbial counts (CFUg-1) were dominated by aerobic mesospheric bacteria count (6.8 ) CFU g-1, S. aurous (4.4 ± 0.5) CFU g-1, Enterobacteriaceae (2.96 ± 0.1 CFU g-1), fungi (2.3 ) CFU g-1,the count of Coli form bacteria were 1.96 log10 CFU g-1 and 3.2 log10 CFU in area 2 and 3 samples, respectively .Among ..the total 120 isolates 18 Fungi and 102 bacterial isolates are identified as bacterial isolates. Bacillus spp. were the most prevalent (43.7%) among others followed by Staphylococcus spp. (24%), Pseudomonas spp. (8%), Acinetobacter spp. (6%) and Coli form (3%) The crude enzyme activity and stability study revealed that the amylase is stable within the pH 6 – 8phvalus and temperature 30˚C - 40˚Cwith the maximum activity at 37˚Ctempereture and 8 pH value. In this study, temperature and moisture content had major roles on the activity of amylase., the microbial quality of soil associated with municipal solid waste of Bedele town was poor, particularly Among the the four isolates BI16,BI5,FIA,FIH exhibited amylase production, FIH selected for amylase production with high titer value. These results have increased the scope of finding industrially important bacteria from municipal waste dump sites and FIH isolates could be vital source for the discovery of industrially useful enzymes. Keywords: waste dump sites, bacteria, , extracellular enzyme