Abstract:
A simple, two-step AuNPs/rGO-MWCNTs nanocomposite fabrication methodology, via drop-casting of rGO-MWCNTs on bare
screen printed electrode (SPE) followed by electrodeposition of AuNPs through voltammetric scanning, without using any toxic
reducing agents is reported. Each step of the synthesized nanocomposite is characterized using state-of-art spectroscopic and
electrochemical methods. Our results demonstrate that the nanostructured AuNPs/rGO-MWCNTs/SPE showed synergistic effects
of high electrocatalytic and electron transfer activity, high surface area to volume ratio, that confirms our synthesized material is the
best material to fabricate a portable device for rapid, inexpensive, and sensitive electrochemical biosensors. For validation, the
fabricated material subjected to electrochemical sensing of BSA protein. Our AuNPs/rGO-MWCNTs nanocomposite exhibited
significant and good linear response over 2.5–12.5 μg ml−1 BSA concentration range, with sensitivity of 1.723 μAμg ml−1 and
limit of detection (LOD) 0.23 μg/ml. This portable sensing system with enhanced performance demonstrates great potential for our
synthesized AuNPs/rGO-MWCNs/SPE nanocomposite in application of point of care applications, and offers a universal and
reliable platform for in biomarker detection.
