Abstract:
Cancer is a serious health problem, with a rising trend in its occurrence documented globally. Female breast cancer is the most
common type of cancer worldwide with treatment consisting of diferent strategies like mastectomy, chemotherapy, and ra
diotherapy. Anticancer property has been studied in withaferin-A (WA), a bioactive compound of Withania somnifera. Another
natural substance derived from bees, propolis, has been investigated for many benefcial efects on human diseases. Te current
study aims to investigate the ameliorative efcacy and antiproliferative potential of combinational drug therapy of withaferin-A
and propolis on breast cancer cells. By evaluating the levels of glycoproteins, nucleic acids, and the marker enzymes in benzo[a]
pyrene-induced breast cancer-bearing female Wistar rats, the pharmacodynamic efects of withaferin-A and propolis drug
combination were examined. Biochemical analysis of DNA, RNA, and protein levels in the liver demonstrated typical results after
propolis therapy. Withaferin-A and propolis drugcombination treatmentsignifcantly decreasednucleic acid synthesis,indicating
that combination chemotherapy has increased breast tumoricidal efciency. Te pharmacological combination therapy exhibited
the capacity to control glycoproteins associated with tumor growth (hexose, hexosamine, and sialic acid), with a considerable
decrease in their levels detected. Histopathological analysis of the mammary glands demonstrated a decrease in hyperplasia and
cell proliferation, indicating that the treatment has the ability to reverse architectural and morphological abnormalities associated
with breast cancer. Tis study found that natural drug compounds in combination have shown regenerative and regulating efects
when given to rats carrying the breast cancer gene.
