Abstract:
In this study, rhodamine B was synthesized and used to modify glassy carbon electrode
for the electrochemical determination of DA. Cyclic voltammetry was used to further analyze the
electrochemical properties of bare glassy carbon electrode and Rhodamine B modified glassy
carbon electrode. It was found that rhodamine B modified glassy carbon electrode exhibited
superior electro catalytic oxidation, enhanced anodic peak current, and less over potential
towards DA as compared to bare glassy carbon electrode and Experimental parameters such as,
scan rate, effect of pH, polymerization of rhodamine B were optimized. The suggested electrode
showed excellent repeatability, reproducibility, and a low detection limit. DA in a urine sample
was successfully determined using the rhodamine B modified glassy carbon electrode. Under the
optimum experimental conditions, the oxidation peak currents had good linear relationship with
DA concentrations in the range of 0.02 - 0.20 μM. In addition, the obtained sensor showed good
sensitivity, favorable repeatability, and long term stability. Finally, the proposed electrochemical
method has been successfully applied for the determination of dopamine in real samples with
satisfactory results.
