Electrochemical Determination of Dopamine at Glassy Carbon Electrode Modified With Rhodamine B

Abstract

In this study, rhodamine B was synthesized and used to modify glassy carbon electrode for the electrochemical determination of DA. Cyclic voltammetry was used to further analyze the electrochemical properties of bare glassy carbon electrode and Rhodamine B modified glassy carbon electrode. It was found that rhodamine B modified glassy carbon electrode exhibited superior electro catalytic oxidation, enhanced anodic peak current, and less over potential towards DA as compared to bare glassy carbon electrode and Experimental parameters such as, scan rate, effect of pH, polymerization of rhodamine B were optimized. The suggested electrode showed excellent repeatability, reproducibility, and a low detection limit. DA in a urine sample was successfully determined using the rhodamine B modified glassy carbon electrode. Under the optimum experimental conditions, the oxidation peak currents had good linear relationship with DA concentrations in the range of 0.02 - 0.20 μM. In addition, the obtained sensor showed good sensitivity, favorable repeatability, and long term stability. Finally, the proposed electrochemical method has been successfully applied for the determination of dopamine in real samples with satisfactory results.