Abstract:
Background:Reference Intervals are the result of quantitative analysis of a clinical chemistry
parameters, obtained from a group of apparently health individual or group of individuals who
are selected according to the clearly defined criteria of International Federation Of Clinical
Chemistry/Clinical Laboratory Science Institute(IFCC/CLSI )recommend. Selecting reference
individuals are an essential but difficult step in the production of RIs throughout the world. As
the diversity of the global population increases, a serious need exists to develop meaningful and
reliable RI that can be used across regions and population groups.
Objective: To determine Reference Interval of selected clinical chemistry parameters for
apparently healthy individuals with age ≥5 years and to show sex and age difference in reference
intervals in south west, Ethiopia.
Methods: A community based cross sectional study was conducted by involving a total of 998
apparently healthy male and female subjects of age 5-71years from the three town with the ratio
of their population and the study was conduct from march 2017-May 2017G.C.A serum sample
was used for clinical chemistry parameters analysis by using HUMANSTAR-100
analyzer(HUMAN, Germany). Control samples were used for every test batch for all parameters.
RIs were constructed using non-parametric methods to estimate 2.5 and 97.5 percentiles of
distribution as lower and upper reference limits, respectively. Data were entered using Epi-data
and analyzed using SPSS version 20 software. Mann Whitney U test and Kruskal Wallis test were
used to show sex and age difference respectively.
Result: There were significant differences in relation to gender in all age group RI for
aspartate aminotransferase, Alanine aminotransferase, alkaline phosphatase, urea and
creatinine. There were also significant age differences between all age group in the RI of both
sex for aspartate aminotransferase and Alanine aminotransferase.
Conclusion:. The findings of this study provide sex and age specific Reference Intervals for
age >=5 years from south west Ethiopian region. Some parameters of this study show sex and
age difference and also a difference between other countries and reagent providers RI. Adoption
of this RI is recommended in this region and determination of similar RI for other regions in
Ethiopia
