Abstract:
Background: Globally, liver disease is the leading cause of death and 50% of liver failure is
caused by drug-induced liver damage. Hepatotoxicity attributed with anti-TB drugs accounts 5–
34.9% TB patients. Many drugs and other therapies are applied to treat liver disease, but the
efficiency and side effect of those drugs are the other burden. Traditionally C.aurea is used to
treat liver disease in different part of Ethiopia. This study aimed to evaluate the effects of
methanolic leaf extracts of C. aurea in INH -RIF induced hepatotoxicity in mice.
Methods: From both sexes 30 mice were randomly divided into six groups. G-I treated with
1mL of normal saline, G-II administered INH 100mg/kg - RIF 100mg/kg, G-III treated with
100mg/kg Silymarin and INH-RIF, G-IV given C. aurea 200mg/kg and INH -RIF, G-V treated
with C. aurea 400mg/kg and INH- RIF and G-VI given 600mg/kg of C. aurea extract with INH
-RIF per day for 21 days. Mice were fasted for 12 hours on 21 th day and anesthetized by
ketamine/xylazine. Then blood sample were taken to determine serum level of liver enzymes,
total bilirubin and albumin; whereas liver was taken for histopathological investigation. Data
were entered into epidata and exported to SPSS for analysis. Statistical comparisons among
groups were done by one way ANOVA followed by Tukey's test at P< 0.05 and expressed as
mean ± SD.
Result: G-II mice showed elevated serum levels of liver enzymes and TBIL with lower albumin
levels than G-I mice. G-V mice (400mg/kg C.aurea extract) showed significant decrease in ALT,
AST, ALP, TBIL and increase in albumin which is comparable to Silymarin group; whereas G-
IV & G-VI shows insignificant reduction in LFT parameters as compared to G-II. The liver
sections of G-II mice displayed lobular lymphocytic inflammation, necrosis, hepatocellular &
canalicular cholestasis, sinusoidal dilatation, and vacuolar degeneration, while these deformities
and abnormalities were not seen in G-V mice.
Conclusion and Recommendation: According to findings C.aurea extract showed good
hepatoprotective potential against INH-RIF-induced liver injury. For other researchers
recommended conducting more studies on hepatoprotective effect of C.aurea on different
models.
