Abstract:
Brucellosis, caused by gram-negative bacteria, is a neglected zoonotic disease affecting
domestic animals and wildlife, particularly prevalent in East Africa, including Ethiopia. This
study aimed to estimate seroprevalence of camel brucellosis, associated risk factors and
assessment of community knowledge about brucellosis in the study area. Across-sectional
study was conducted from January to December 2024 in the Gomole and Elwaye Districts of
Borena Zone, Southern Ethiopia. A total of 384 camels from 74 house hold (herds) were
randomly sampled. Serum samples were screened using the Rose Bengal plate test, with
positive cases confirmed by an Indirect Enzyme-Linked Immunosorbent Assay (ELISA). The
overall prevalence of camel Brucellosis was found to be 2.86%with 95% and (CI: 1.6-5.1)
(3.6% in Elwaye and 2.1% in Gomole), with a herd-level prevalence of 13.51%with 95% and
(CI:7.5-23.1).
