Abstract:
Escherichia coli and Salmonella species that were distributed widely contribute to risein resistance to
various antibiotics used commercially with problem to health as uses of antimicrobials have critical
importance for emergence of resistance bacteria.A cross-sectional study was conducted from
November 2018 to December 2019 to assess prevalence and antibiotic resistance profile of
Escherichia coliand Salmonellaspecies from samples in dairy farms with survey on perception
antimicrobial use, resistance and public health aspect in selected districts of western Oromia,
Ethiopia.Of 60 respondents were interviewed and subsequently, 384 samples collected consisting cow
milk, fecal, bucket milk and bucket swab for isolation and identificationby biochemical tests, SPSS
analyzed andChi-square used to assess association of bacteria in samples.Isolated bacteriasubjected
susceptibility test using 8 antibiotics by using disk diffusion test.From overall prevalence observed
63(16.4%) for Escherichia coli and 31(8.1%) Salmonella.Significantly high proportion of Escherichia
coli (19.2%) and Salmonella species (10.5%) obtained in udder milk indicated (p= 0.016, 0.004); 5%
and 5% low proportion in bucket swab and bucket milk, respectively. High prevalence of Escherichia
coli (15.7%) and Salmonella species (6.4%)was observed in feces of cattle. Escherichia coli and
Salmonella isolate result of disk diffusion was significantly resistant to cefoxitin (71.4%, 77.4%),
tetracycline (65.8%, 67.8%), streptomycin (55.6%, 61.3%), for ciprofloxacin and gentamycin greater
than 50%. Isolates, 90.5% Escherichia coli and 87.1% Salmonella species isolate resistant to multiple
antibioticswere used. About, (84.9%, 83.3%), and (100%, 90.9%) Escherichia coli and Salmonella
species isolates from milk and feces respectively were multi-drug resistant. Study bacteriawere isolates
susceptible to nitrofurantoin, nalidixic acid, ceftriaxone and ciprofloxacin. Of respondents, yet
worryingly 86.7% knew what antibiotics and 88.3% believed waste management not causes antibiotic
resistance. Samples harbored targeted study bacteria and antibiotic resistant profile of
isolatedEscherichia coli and Salmonella that have health hazard. Therefore, strengthening of
communitiesto minimize malpractice of antibiotic useswas importance to limit associated health risk
and occurrence of antimicrobial resistance in study areas.
